Effects of climate warming on rice quality in relation to hybrid combinations and their parents
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摘要:
探明气候、杂交组合与亲本对稻米品质的互作效应, 可为进一步优化水稻品质改良策略和提升水稻防灾减灾能力提供科学依据。本文于2021年和2022年以3个不育系和5个恢复系配组的15个杂交组合为试验材料, 在早季常温和晚季高温2种生态条件下进行裂区试验, 研究了杂交组合及其亲本对稻米品质的影响。结果表明, 年度间8项稻米品质的差异均达极显著水平。与2022年相比, 2021年水稻长宽比、垩白度、蛋白质、直链淀粉、胶稠度、精米率和整精米率分别显著提高8.41%、99.36%、13.76%、2.54%、125.12%、11.92%和144.00% (P<0.05), 但垩白粒率显著降低37.91% (P<0.05)。就不同种植季节来看, 晚季的长宽比、蛋白质和整精米率分别比早季显著提高3.96%、7.34%和14.08% (P<0.05), 而晚季垩白粒率、胶稠度和精米率则分别显著下降15.43%、5.21%和1.85%, 垩白度和直链淀粉在两个季节间差异不显著。不同不育系和不同恢复系分别配制的杂交组合间各品质指标差异均达显著水平, 3个不育系配制的杂交组合中‘椰香A’的综合品质相对较好, 5个恢复系配制的杂交组合中‘泸恢127’和‘泸恢276’的综合品质相对较好。由2021年和2022年合计分析结果可见, 两个亲本对长宽比、垩白度、垩白粒率、蛋白质和直链淀粉的特殊配合力方差较高(52.07%~92.39%), 对胶稠度、精米率和整精米率的一般配合力方差较高(73.90%~86.20%), 8项品质指标的广义遗传力均较高(48.29%~98.01%); 早季与晚季长宽比、垩白粒率、蛋白质、胶稠度、精米率和整精米率差异均达显著或极显著水平, 且早季与晚季间呈极显著正相关, 垩白度虽然在早季与晚季间差异不显著, 但两季间仍呈极显著正相关。综上, 未来杂交水稻优质育种策略是选择垩白度低, 胶稠度、整精米率和垩白粒率高的恢复系, 以及长宽比大、垩白度和垩白粒率低、蛋白质含量8%左右和直链淀粉含量16%~18%的不育系杂交, 并从杂交后代中选择优质杂交稻品种。
Abstract:The study of the interaction effects of climate, hybrid combination, and parents on rice quality can provide a scientific basis for further optimization of rice quality improvement strategies and improvement of rice disaster prevention and mitigation abilities. In 2021 and 2022, 15 hybrid combinations, obtained using three sterile lines and five restorer lines, were used as experimental materials under two ecological conditions (normal temperature in the early season and high temperature in the late season), and the responses of rice quality to hybrid combinations and their parents were investigated. The results showed that the differences in the eight rice qualities were all very significant. The ratio of length to width, chalkiness, protein, amylose, gel consistency, milled rice rate, and head rice rate in 2021 were 8.41%, 99.36%, 13.76%, 2.54%, 125.12%, 11.92%, and 144.00% (P<0.05) higher than those in 2022, respectively, whereas the chalky rice rate was 37.91% (P<0.05) lower than that in 2022. The ratio of length to width and protein and head rice rates in the late season significantly increased by 3.96%, 7.34%, and 14.08% (P<0.05), respectively, compared to those in the early season. The chalky rice rate, gel consistency, and milled rice rate in the late season significantly decreased by 15.43%, 5.21%, and 1.85% compared with those in the early season, respectively; however, there was no significant difference between chalkiness and amylose in the two seasons. The quality indices of the hybrid combinations prepared using different sterile lines and restorer lines were significantly different. The comprehensive quality of ‘Yexiang A’ in the hybrid combinations prepared by three sterile lines was relatively good. The comprehensive quality of ‘Luhui 127’ and ‘Luhui 276’ in the hybrid combination of the five restorer lines was relatively good. The variance of the special combining ability of parents for the ratio of length to width, chalkiness, chalky rice rate, protein, and amylose was very high (52.07%–92.39%), and the variance of general combining ability for gel consistency, milled rice rate, and head rice rate was very high (73.90%–86.20%). The broad sense heritability of eight quality indexes was high (48.29%–98.01%). There were significant or extremely significant differences in the ratio of length to width, chalky rice rate, protein, gel consistency, milled rice rate, and head rice rate between early season and late season, and there was a very significant positive correlation between early season and late season. Although there was no significant difference in the chalkiness between the early and late seasons, there was a significant positive correlation between them. In summary, the future breeding strategy for hybrid rice quality is to select restorer lines with low chalkiness, high gel consistency, head rice rate, and chalky rice rate, cross them with sterile lines with high ratio of length to width, low chalkiness and chalky rice rate, approximately 8% protein content, and 16%–18% amylose content, and select high-quality hybrid rice varieties from the hybrid offspring.
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Keywords:
- Climate warming /
- Hybrid rice /
- Rice quality /
- Hybrid parents /
- Breeding strategy
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表 1 试验地土壤背景值
Table 1 Soil background value of the experimental soil
年度
YearpH 有机碳
Organic
carbon
(g·kg−1)有机质
Organic
matter
(g·kg−1)全氮
Total
nitrogen
(%)全磷
Total
phosphorus
(g·kg−1)全钾
Total
potassium
(g·kg−1)水解性氮
Hydrolyzed
nitrogen
(mg·kg−1)有效磷
Available
phosphorus
(mg·kg−1)速效钾
Rapidly available
potassium
(mg·kg−1)2021 5.78 20.4 35.2 0.114 0.41 15.6 68.2 1.7 121 2022 5.37 19.2 33.2 0.185 0.57 17.1 122.0 1.0 153 表 2 2021年不同种植季节杂交组合间稻米品质多重比较
Table 2 Multiple comparison of rice quality among hybrid combinations in different planting seasons in 2021
季节
Season杂交组合
Cross combination长宽比
Ratio of length to width垩白度
Chalkiness
(%)垩白粒率
Chalky rice rate
(%)蛋白质
Protein
(%)直链淀粉
Amylose
(%)胶稠度
Gel consistency
(mm)精米率
Milled rice rate
(%)整精米率
Head rice rate
(%)早季
Early
season椰香A/泸恢107
Yexiang A / Luhui 1073.43±0.12ab 28.73±6.59abcd 16.67±7.02abc 8.23±0.17ab 15.17±1.19abc 84.00±3.78ab 75.70±0.26a 40.27±6.76ab 椰香A/泸恢127
Yexiang A / Luhui 1273.63±0.06a 28.60±4.54abcd 18.00±4.00abc 7.76±0.11b 14.83±1.79abc 83.37±3.34b 74.83±1.66ab 41.63±1.63ab 椰香A/泸恢276
Yexiang A / Luhui 2763.47±0.06ab 19.63±0.90cd 17.33±5.03abc 7.70±0.10b 15.87±0.90abc 85.23±4.49ab 72.87±2.51abc 41.20±2.74ab 椰香A/泸恢1156
Yexiang A / Luhui 11563.50±0.10ab 18.17±0.51d 14.67±2.31abc 7.77±0.13ab 13.97±0.84bc 83.63±3.16b 72.83±2.32abc 38.47±3.40b 椰香A/泸恢6150
Yexiang A / Luhui 61503.60±0.10a 20.23±0.12bcd 11.33±5.03bc 8.41±0.30ab 14.00±0.53bc 95.00±1.08ab 73.47±1.86abc 45.00±2.95ab 沪旱82S/泸恢107
Huhan 82S / Luhui 1073.40±0.10ab 16.87±2.27d 10.00±3.46c 8.33±0.14ab 17.50±0.52a 92.03±4.60ab 70.87±1.59abc 38.30±2.77b 沪旱82S/泸恢127
Huhan 82S / Luhui 1273.37±0.06abc 27.20±2.65abcd 18.00±2.00abc 8.05±0.17ab 16.80±0.62ab 90.67±6.01ab 71.53±1.18abc 44.13±1.76ab 沪旱82S/泸恢276
Huhan 82S / Luhui 2763.07±0.12c 19.23±1.10cd 12.00±2.00bc 7.76±0.15b 16.83±1.10ab 83.60±4.01b 72.30±1.57abc 42.77±2.61ab 沪旱82S/泸恢1156
Huhan 82S / Luhui 11563.33±0.06abc 27.57±5.77abcd 16.67±1.15abc 8.00±0.22ab 15.90±1.25abc 85.93±1.96ab 72.00±0.30abc 42.50±1.56ab 沪旱82S/泸恢6150
Huhan 82S / Luhui 61503.27±0.06bc 23.30±1.95abcd 15.33±5.03abc 8.13±0.21ab 15.43±1.33abc 94.33±7.15ab 72.00±2.02abc 47.30±5.37ab 川康606A/泸恢107
Chuankang 606A / Luhui 1073.37±0.06abc 28.47±3.33abcd 23.33±9.24abc 8.66±0.41a 15.33±0.25abc 88.77±3.65ab 68.43±2.90c 43.83±5.75ab 川康606A/泸恢127
Chuankang 606A / Luhui 1273.40±0.10ab 34.20±10.44ab 28.00±6.93ab 7.60±0.15b 15.10±1.39abc 95.67±3.37a 72.60±0.61abc 44.53±2.66ab 川康606A/泸恢276
Chuankang 606A / Luhui 2763.20±0.20bc 36.13±5.61a 22.67±4.16abc 8.00±0.23ab 16.57±1.00abc 83.47±1.51b 72.23±1.21abc 38.87±2.41ab 川康606A/泸恢1156
Chuankang 606A / Luhui 11563.47±0.15ab 32.63±4.84abc 29.33±6.43a 7.87±0.19ab 13.53±1.72c 90.30±3.14ab 70.57±1.33bc 39.03±2.27ab 川康606A/泸恢6150
Chuankang 606A / Luhui 61503.40±0.17ab 28.60±7.28abcd 24.67±10.07abc 8.07±0.85ab 14.43±0.21abc 87.23±2.21ab 74.77±1.59ab 49.23±3.21a 平均 Mean 3.39 25.97 18.53 8.03 15.42 88.22 72.47 42.47 CV (%) 4.21 23.38 31.78 3.68 7.58 5.12 2.52 8.05 F值 F value 5.64** 4.83** 3.38** 2.94** 3.49** 4.05** 3.46** 2.59** 晚季
Late
season椰香A/泸恢107
Yexiang A / Luhui 1073.77±0.21ab 18.30±5.82b 11.67±2.08abc 9.04±0.23a 16.33±0.91ab 82.10±2.29d 76.67±1.06a 54.67±2.90abc 椰香A/泸恢127
Yexiang A / Luhui 1273.83±0.06a 24.53±6.67b 13.67±2.08abc 8.71±0.30a 15.20±1.35ab 96.37±2.44a 74.53±2.37a 51.27±1.02abcd 椰香A/泸恢276
Yexiang A / Luhui 2763.67±0.06abcd 24.00±3.54b 12.00±2.00abc 8.45±0.04ab 17.63±1.24a 96.93±4.03a 75.80±0.80a 58.70±1.70a 椰香A/泸恢1156
Yexiang A / Luhui 11563.73±0.06abc 15.40±1.82b 7.33±1.15c 8.68±0.17a 15.20±0.79ab 83.97±3.67cd 75.83±0.06a 53.30±4.33abcd 椰香A/泸恢6150
Yexiang A / Luhui 61503.70±0.10abc 17.43±5.32b 9.33±3.06bc 8.55±0.24ab 15.60±0.30ab 95.40±2.86ab 75.10±1.31a 55.23±5.01ab 沪旱82S/泸恢107
Huhan 82S / Luhui 1073.57±0.06abcde 24.50±1.44b 7.34±1.14c 8.74±0.24a 14.20±0.87b 96.17±3.23a 75.40±0.46a 51.57±6.47abcd 沪旱82S/泸恢127
Huhan 82S / Luhui 1273.47±0.06bcde 19.10±4.54b 10.67±2.31abc 8.44±0.30ab 14.90±0.61ab 90.23±2.64abcd 75.43±0.25a 51.00±9.40abcd 沪旱82S/泸恢276
Huhan 82S / Luhui 2763.37±0.12de 29.53±5.77ab 12.67±2.31abc 7.98±0.13ab 15.77±1.29ab 82.90±1.56cd 76.30±0.35a 52.27±4.38abcd 沪旱82S/泸恢1156
Huhan 82S / Luhui 11563.47±0.15bcde 29.47±2.90ab 18.33±1.53a 8.16±0.07ab 15.17±0.96ab 92.57±3.88abcd 75.80±0.56a 54.70±2.61abc 沪旱82S/泸恢6150
Huhan 82S / Luhui 61503.60±0.10abcde 18.97±2.83b 9.33±1.15bc 8.84±0.25a 15.63±0.64ab 86.83±2.68abcd 73.13±0.90ab 58.50±8.40a 川康606A/泸恢107
Chuankang 606A / Luhui 1073.50±0.10bcde 16.57±5.49b 8.67±4.62bc 8.90±0.08a 17.30±1.23a 88.17±2.27abcd 69.40±1.00bc 40.20±11.06bcd 川康606A/泸恢127
Chuankang 606A / Luhui 1273.43±0.12cde 29.87±2.80ab 7.67±1.53c 8.39±0.24ab 16.70±0.66ab 94.97±3.61ab 68.00±2.95c 38.93±1.10cd 川康606A/泸恢276
Chuankang 606A / Luhui 2763.30±0.00e 42.50±13.62a 16.67±6.43ab 8.27±0.82ab 17.57±1.52a 85.83±3.76bcd 74.33±1.11a 54.83±2.87abc 川康606A/泸恢1156
Chuankang 606A / Luhui 11563.50±0.00bcde 29.43±6.05ab 14.67±3.06abc 7.60±0.14b 16.77±0.86ab 92.13±1.27abcd 68.10±1.92c 37.60±3.86d 川康606A/泸恢6150
Chuankang 606A / Luhui 61503.57±0.12abcde 20.90±1.21b 8.67±1.15bc 8.89±0.85a 17.10±0.53ab 89.90±6.83abcd 68.40±1.55c 40.87±2.96bcd 平均 Mean 3.57 24.03 11.24 8.50 16.07 90.30 73.48 50.24 CV (%) 4.26 30.06 30.47 4.58 6.63 5.69 4.42 14.25 F值 F value 6.55** 5.15** 4.61** 3.52** 3.58** 6.93** 17.21** 5.27** 同季同列不同小写字母表示不同处理间在P<0.05水平差异显著, **表示在P<0.01水平差异显著。Different lowercase letters in the same column in the same season indicate significant differences at P<0.05 level, and ** indicates significant difference at P<0.01 level. 表 3 2022年不同种植季节杂交组合间稻米品质多重比较
Table 3 Multiple comparison of rice quality among hybrid combinations in different planting seasons in 2022
季节
Season杂交组合
Cross combination长宽比
Ratio of length to width垩白度
Chalkiness
(%)垩白粒率
Chalky rice rate
(%)蛋白质
Protein
(%)直链淀粉
Amylose
(%)胶稠度
Gel consistency
(mm)精米率
Milled rice rate
(%)整精米率
Head rice rate
(%)早季
Early
season椰香A/泸恢107
Yexiang A / Luhui 1073.40±0.10a 5.60±0.76f 19.33±19.33bcde 7.26±0.41ab 15.33±0.50b 40.23±4.52bcd 67.57±0.55ab 19.10±1.59ab 椰香A/泸恢127
Yexiang A / Luhui 1273.33±0.06ab 8.73±2.82ef 11.67±11.67de 7.06±0.18abc 15.10±0.62b 39.77±4.00bcd 68.40±2.21ab 21.83±2.57ab 椰香A/泸恢276
Yexiang A / Luhui 2763.23±0.15bc 4.87±2.24f 11.00±11.00e 6.44±0.09c 15.00±0.78b 36.33±4.04cd 69.40±1.40ab 19.60±2.11ab 椰香A/泸恢1156
Yexiang A / Luhui 11563.23±0.15abc 12.00±1.87de 33.00±33.00ab 6.68±0.01bc 15.50±0.56b 46.17±5.01abcd 68.73±3.96ab 11.47±0.65c 椰香A/泸恢6150
Yexiang A / Luhui 61503.33±0.23ab 4.03±1.62f 11.00±11.00e 7.06±0.19abc 15.67±0.32ab 42.17±0.29abcd 69.80±1.31a 21.00±1.95ab 沪旱82S/泸恢107
Huhan 82S / Luhui 1073.37±0.12ab 17.63±2.89bcd 35.67±35.67a 6.83±0.24abc 15.27±0.21b 34.63±1.52d 63.50±2.96b 12.00±1.65c 沪旱82S/泸恢127
Huhan 82S / Luhui 1272.97±0.06bc 25.07±1.10a 29.00±29.00abc 6.62±0.20bc 15.23±0.29b 52.23±5.65abc 67.30±0.95ab 19.30±2.00ab 沪旱82S/泸恢276
Huhan 82S / Luhui 2762.83±0.06c 26.73±3.33a 35.67±35.67a 6.45±0.03c 15.67±0.15ab 57.67±6.35a 68.33±1.06ab 22.60±0.36a 沪旱82S/泸恢1156
Huhan 82S / Luhui 11563.20±0.17abc 18.37±1.80b 32.67±32.67ab 6.76±0.15abc 15.20±0.36b 42.73±3.93abcd 65.50±2.00ab 16.03±0.81bc 沪旱82S/泸恢6150
Huhan 82S / Luhui 61503.10±0.17abc 17.73±0.31bc 24.33±24.33abcde 6.97±0.05abc 15.07±0.40b 43.33±8.09abcd 65.40±1.25ab 20.50±1.95ab 川康606A/泸恢107
Chuankang 606A / Luhui 1073.23±0.15abc 14.17±0.67bcde 32.00±32.00ab 7.00±0.22abc 15.30±0.56b 35.67±6.03cd 64.57±1.02ab 17.23±2.87abc 川康606A/泸恢127
Chuankang 606A / Luhui 1273.13±0.12abc 12.17±0.68cde 26.67±26.67abcd 7.13±0.53abc 15.73±0.21ab 41.67±8.96abcd 67.40±3.22ab 21.30±3.49ab 川康606A/泸恢276
Chuankang 606A / Luhui 2763.10±0.17abc 4.57±2.42f 9.00±9.00e 7.51±0.29a 16.27±0.47ab 54.57±6.08ab 67.00±0.26ab 19.93±2.16ab 川康606A/泸恢1156
Chuankang 606A / Luhui 11563.00±0.10abc 14.07±1.31bcde 29.00±29.00abc 7.23±0.19ab 16.97±0.72a 50.93±5.25abcd 66.60±3.36ab 18.53±1.48ab 川康606A/泸恢6150
Chuankang 606A / Luhui 61503.17±0.06abc 3.07±1.22f 13.67±13.67cde 7.50±0.42a 15.70±0.26ab 44.07±7.75abcd 65.40±1.40ab 18.40±1.13ab 平均 Mean 3.17 12.59 23.58 6.97 15.53 44.14 66.99 18.59 CV (%) 5.07 59.58 42.28 4.88 3.33 15.77 2.72 17.75 F值 F value 4.14** 47.87** 11.38** 5.17** 3.68** 4.59** 2.28* 8.35** 晚季
Late
season椰香A/泸恢107
Yexiang A / Luhui 1073.40±3.40ab 8.40±2.60ef 20.00±5.29bcde 7.76±0.09ab 14.97±0.40ab 34.20±1.93ab 66.63±1.20a 16.87±1.82bc 椰香A/泸恢127
Yexiang A / Luhui 1273.43±3.43ab 13.47±1.55cde 23.00±6.08bcde 7.55±0.18ab 14.93±0.25ab 38.00±2.00ab 67.10±4.08a 20.23±1.57abc 椰香A/泸恢276
Yexiang A / Luhui 2763.17±3.17abc 4.17±2.31f 10.67±1.53e 7.54±0.21ab 15.40±0.30ab 35.50±4.27ab 67.67±1.70a 19.73±2.84abc 椰香A/泸恢1156
Yexiang A / Luhui 11563.33±3.33abc 13.53±0.42cde 33.67±4.62abc 7.31±0.12b 15.53±0.25ab 37.67±3.06ab 64.73±2.42abc 17.33±2.08bc 椰香A/泸恢6150
Yexiang A / Luhui 61503.53±3.53a 3.67±1.75f 12.00±2.65e 7.87±0.26ab 15.00±0.30ab 31.47±0.85b 65.93±0.47ab 21.37±1.12ab 沪旱82S/泸恢107
Huhan 82S / Luhui 1073.10±3.10bc 26.30±2.67ab 43.67±4.73a 7.39±0.29ab 15.13±0.49ab 32.30±0.80ab 62.37±2.06abcd 15.80±0.85c 沪旱82S/泸恢127
Huhan 82S / Luhui 1273.23±3.23abc 22.57±4.97bc 38.00±3.46ab 7.40±0.41ab 14.53±0.40b 39.40±3.33a 58.97±2.82cd 17.30±1.15bc 沪旱82S/泸恢276
Huhan 82S / Luhui 2762.97±2.97c 11.17±3.42def 23.33±6.66bcde 7.25±0.21b 15.90±0.10a 32.30±3.04b 63.10±1.68abcd 21.30±1.37ab 沪旱82S/泸恢1156
Huhan 82S / Luhui 11563.07±3.07bc 31.73±2.06a 44.33±10.79a 7.33±0.24b 14.93±0.40ab 32.00±1.05b 57.93±3.10d 15.80±1.50c 沪旱82S/泸恢6150
Huhan 82S / Luhui 61503.10±3.10bc 18.83±2.76bcd 32.33±10.50abcd 7.77±0.22ab 14.53±0.47b 32.07±1.68ab 60.03±1.53bcd 20.93±1.59abc 川康606A/泸恢107
Chuankang 606A / Luhui 1073.30±3.30abc 9.30±1.57ef 21.67±2.31bcde 8.12±0.26a 15.30±0.66ab 38.00±3.20ab 65.17±1.99abc 21.77±0.95ab 川康606A/泸恢127
Chuankang 606A / Luhui 1273.37±3.37ab 7.10±3.94ef 17.00±7.00cde 7.62±0.47ab 14.90±0.78ab 33.40±3.05ab 63.70±0.76abcd 23.73±2.29a 川康606A/泸恢276
Chuankang 606A / Luhui 2763.07±3.07bc 5.93±3.41ef 16.67±5.86cde 7.75±0.34ab 15.37±0.06ab 34.37±2.57ab 59.97±1.12bcd 18.83±1.40abc 川康606A/泸恢1156
Chuankang 606A / Luhui 11563.33±3.33abc 6.50±4.60ef 14.00±5.29e 7.46±0.15ab 15.77±0.55ab 39.47±3.54a 63.37±2.84abcd 20.50±2.72abc 川康606A/泸恢6150
Chuankang 606A / Luhui 61503.33±3.31abc 4.60±3.90ef 15.33±5.51de 7.39±0.15ab 15.40±0.61ab 37.33±3.92ab 64.33±0.99abcd 19.73±1.95abc 平均 Mean 3.25 12.48 24.38 7.57 15.17 35.16 63.40 19.41 CV (%) 4.98 69.43 46.27 3.22 2.65 7.74 4.78 12.09 F值 F value 5.29** 24.29** 10.44** 2.65** 2.42* 3.30** 6.00** 5.26** 同季同列不同小写字母表示不同处理间在P<0.05水平差异显著, *和**分别表示在P<0.05和P<0.01水平差异显著。Different lowercase letters in the same column in the same season indicate significant differences at P<0.05 level. * and ** indicate significant differences at P<0.05 and P<0.01 levels, respectively. 表 4 水稻早季与晚季生长期间气象条件比较
Table 4 Comparison of meteorological conditions between early and late rice growing periods
年度
Year季节
Season全生育期气象条件
Meteorological conditions of the whole growth period灌浆期气温
Temperature during grain filling period日平均气温
Daily mean temperature
(℃)日最高气温
Daily maximum temperature
(℃)日最低气温
Daily minimum temperature
(℃)日照
时数
Sunlight
hour
(h)降水
Precipitation
(mm)日均相
对湿度
Daily relative humidity
(%)日平均气温
Daily mean temperature
(℃)日最高气温
Daily maximum temperature
(℃)日最低气温
Daily minimum temperature
(℃)2021 早季
Early season23.44 27.95 20.33 563.65 582.40 76.39 29.31 34.23 25.91 晚季
Late season26.53 31.01 23.47 503.05 733.60 77.67 25.78 30.19 22.87 早季−晚季
Early season−
late season−3.09 −3.06 −3.14 60.60 −151.20 −1.28 3.53 4.04 3.04 2022 早季
Early season24.06 29.04 20.47 798.80 808.70 75.30 30.48 35.94 26.25 晚季
Late season28.54 33.43 25.02 733.20 510.00 69.55 29.94 35.21 25.78 早季−晚季
Early season−
late season−4.48 −4.39 −4.55 65.60 298.70 5.75 0.54 0.73 0.47 表 5 不同年度、季节、不育系和恢复系对杂交组合的稻米品质互作影响
Table 5 Effects of different years, seasons, sterile lines and restorer lines on rice quality of hybrid combinations
项目 Item 长宽比
Ratio of length to width垩白度
Chalkiness (%)垩白粒率
Chalky rice rate (%)蛋白质
Protein (%)直链淀粉
Amylose (%)胶稠度
Gel consistency (mm)精米率
Milled rice rate (%)整精米率
Head rice rate (%)年份
Year (A)2021 3.48±0.19a 25.00±7.81a 14.89±6.95b 8.27±0.50a 15.74±1.42a 89.26±5.67a 72.97±2.90a 46.36±7.65a 2022 3.21±0.19b 12.54±8.16b 23.98±11.32a 7.27±0.47b 15.35±0.61b 39.65±7.78b 65.20±3.49b 19.00±3.19b 季节
Season (B)早季
Early season3.28±0.21b 19.28±9.93a 21.06±9.43a 7.49±0.65b 15.48±1.12a 66.18±23.22a 69.73±3.63a 30.53±12.64b 晚季
Late season3.41±0.24a 18.26±10.30a 17.81±11.14b 8.04±0.63a 15.62±1.10a 62.73±28.13b 68.44±6.10b 34.83±16.67a 不育系
Sterile line (C)椰香A
Yexiang A3.49±0.22a 14.68±8.51c 15.87±7.73c 7.79±0.72a 15.31±1.02b 63.38±26.10b 71.18±4.20a 33.41±15.84a 沪旱82S
Huhan 82S3.24±0.23c 22.59±5.88a 23.47±12.65a 7.66±0.71b 15.48±1.00b 64.70±26.20ab 68.36±6.00b 33.23±16.26a 川康606A
Chuankang 606A3.31±0.18b 19.04±13.12b 18.97±8.93b 7.85±0.65a 15.86±1.23a 65.30±25.43a 67.72±4.08b 31.40±12.51b 恢复系
Restorer line (D)泸恢107
Luhui 1073.40±0.19a 17.90±8.16b 20.83±12.01ab 8.02±0.77a 15.59±1.11b 62.19±27.15b 68.86±4.96ab 30.97±15.39bc 泸恢127
Luhui 1273.38±0.23a 21.05±9.43a 20.11±9.61b 7.69±0.65b 15.33±1.02b 66.31±26.70a 69.15±5.11ab 32.93±13.28ab 泸恢276
Luhui 2763.20±0.24b 19.04±13.46ab 16.64±8.08c 7.59±0.66b 16.15±1.10a 64.06±24.24ab 69.94±5.00a 34.22±15.23a 泸恢1156
Luhui 11563.35±0.22a 20.74±9.12a 23.97±11.58a 7.57±0.57b 15.37±1.19b 64.79±24.34ab 68.50±5.49b 30.44±15.19c 泸恢6150
Luhui 61503.39±0.23a 15.11±8.99c 15.61±8.49c 7.96±0.72a 15.30±0.90b 64.93±27.55a 68.98±4.82ab 34.84±15.66a F值
F valueA 230.40** 422.66** 147.06** 511.33** 10.82** 6723.28** 795.91** 2768.61** B 48.40** 2.83 18.74** 151.62** 1.52 32.50** 21.86** 68.38** C 69.32** 57.04** 34.66** 6.41** 7.30** 3.52* 59.53** 6.15** D 17.64** 12.65** 16.18** 17.78** 7.22** 4.96** 3.01* 11.14** A×B 7.74** 2.29** 29.12** 1.66 18.16** 83.60** 69.87** 44.61** A×C 2.60 90.02** 64.44** 8.04** 1.66 0.70 21.50** 19.54** A×D 0.92 8.94** 12.20** 2.65* 5.76** 7.99** 0.23 1.96 B×C 0.36 5.23** 19.41** 4.55** 16.73** 7.29** 5.15** 13.32** B×D 0.88 1.73 0.22 1.16 1.51 2.54* 4.73** 3.42** C×D 1.47 2.99** 0.60 2.19* 0.14 2.23* 1.17 1.75 A×B×C 5.28** 1.60 0.14 1.40 22.10** 0.60 18.66** 20.82** A×B×D 3.24 ** 8.32** 2.10 0.94 2.28 5.62** 3.67** 4.04** A×C×D 0.82 5.18** 4.69** 1.54 1.69 10.64** 3.84** 2.56** B×C×D 1.68 4.93** 4.22** 1.70 1.12 5.48** 0.69 1.88 A×B×C×D 1.47 2.77** 2.15* 2.84** 0.92 1.13 2.78** 2.35* 同列不同小写字母表示不同年、不同季和不同处理间在P<0.05水平差异显著, *和**分别表示在P<0.05和P<0.01水平差异显著。Different lowercase letters in the same column indicate significant differences between different years, different seasons and different treatments at P<0.05 level. * and ** indicate significant differences at P<0.05 and P<0.01 levels, respectively. 表 6 不育系与恢复系不完全双列杂交对稻米品质的遗传分析
Table 6 Genetic analysis of hybrid rice quality by incomplete diallel crossing between sterile line and restorer line
年份
Year项目
Item变异来源
Variation source长宽比
Ratio of length to width垩白度
Chalkiness垩白粒率
Chalky rice rate蛋白质
Protein直链淀粉
Amylose胶稠度
Gel consistency精米率
Milled rice rate整精米率
Head rice rate2021 F值
F value杂交组合
Cross combination8.13** 4.81** 6.32** 5.02** 3.66** 5.44** 9.09** 6.83** P1 2.40 0.69 2.94* 1.49 2.89* 0.36 7.31** 3.68* P2 0.20 0.15 0.06 0.16 0.73 1.65 0.87 0.55 P1×P2 7.70** 5.93** 5.72** 5.45** 3.16** 5.31** 4.92** 5.28** 配合力方差
Combining ability variance一般配合力
General combining ability (%)21.18 0.00 28.12 9.06 26.63 13.83 56.89 35.48 特殊配合力
Special combining ability (%)78.82 100.00 71.88 90.94 73.37 86.17 43.11 64.52 遗传力
Heritability (%)广义
Broad sense73.92 62.18 68.63 61.97 49.56 62.52 75.18 68.88 狭义
Narrow sense15.66 0.00 19.30 5.61 13.20 8.64 42.77 24.44 2022 F值
F value杂交组合
Cross combination4.78** 29.49** 11.52** 19.15** 3.34** 8.11 6.64** 7.28 P1 1.62 3.52* 2.87* 13.02** 1.08 4.40 9.14** 1.57 P2 0.12 0.22 0.29 0.36 0.76 1.59 1.32 1.81 P1×P2 5.12** 24.31** 10.14** 7.52** 3.44** 5.16 3.05** 5.97 配合力方差
Combining ability variance一般配合力
General combining ability (%)11.32 30.48 25.71 69.79 1.95 45.89 67.87 23.63 特殊配合力
Special combining ability (%)88.68 69.52 74.29 30.21 98.05 54.11 32.13 76.37 遗传力
Heritability (%)广义
Broad sense60.75 91.79 80.40 84.36 45.31 71.95 68.03 68.47 狭义
Narrow sense6.88 27.97 20.67 58.87 0.88 33.02 46.17 16.18 合计
TotalF值
F value杂交组合
Cross combination9.84** 16.49** 11.01** 15.33 3.76 125.62** 20.29** 56.12** P1 10.35** 5.85** 3.74** 8.31 1.71 43.65** 58.26** 33.13** P2 0.32 1.48 0.60 0.27 1.04 0.76 0.51 0.37 P1×P2 6.53** 11.63** 9.91** 11.28 3.59 32.67** 4.24** 18.33** 配合力方差
Combining ability variance一般配合力
General combining ability (%)47.93 35.37 20.24 40.05 7.63 78.57 86.20 73.90 特殊配合力
Special combining ability (%)52.07 64.63 79.76 59.95 92.39 21.43 13.80 26.10 遗传力
Heritability (%)广义
Broad sense77.97 84.58 78.83 85.11 48.29 98.01 88.66 95.68 狭义
Narrow sense52.07 29.91 15.96 34.08 3.69 77.01 76.42 70.71 P1: 恢复系; P2: 不育系。*和**分别表示在P<0.05和P<0.01水平差异显著。P1: restorer line; P2: sterile line. * and ** indicate significance at P<0.05 and P<0.01 levels, respectively. 表 7 15个品种晚季与早季稻米品质的差异比较
Table 7 Comparison of rice quality between late season and early season of 15 varieties
年份
Year项目
Item长宽比
Ratio of length to width垩白度
Chalkiness
(%)垩白粒率
Chalky rice rate
(%)蛋白质
Protein
(%)直链淀粉
Amylose
(%)胶稠度
Gel consistency
(mm)精米率
Milled rice rate
(%)整精米率
Head rice rate
(%)2021 早季
Early season3.39 25.97 18.53 8.02 15.42 88.22 72.47 42.47 晚季
Late season3.57 24.03 11.25 8.51 16.07 90.30 73.48 50.24 早季−晚季
Early season−late season−0.18 1.94 7.28 −0.49 −0.65 −2.08 −1.01 −7.77 t值
t value6.80** 1.12 4.43** 5.24** 1.44 1.55 1.22 3.59** 2季间的r
The r between two seasons0.7826** 0.5054 0.1440 0.4743 −0.2414 0.4245 0.2890 −0.1767 2022 早季
Early season3.17 12.59 23.58 6.97 15.53 44.14 66.99 18.59 晚季
Late season3.25 12.48 24.38 7.57 15.17 35.17 63.40 19.41 早季−晚季
Early season−late season−0.08 0.11 −0.80 −0.60 0.36 8.97 3.59 −0.82 t值
t value2.79* 0.0585 0.3485 7.14** 3.15** 4.81** 5.15** 1.30 2季间的r
The r between two seasons0.5222* 0.6560** 0.6564** 0.4079* 0.5590* 0.1131 0.4715 0.6573 合计
Total早季
Early season3.28 19.28 21.06 7.49 15.48 66.18 69.73 30.53 晚季
Late season3.41 18.26 17.81 8.04 15.62 62.73 68.44 34.83 早季−晚季
Early season−late season−0.13 1.02 3.25 −0.55 −0.14 3.45 1.29 −4.30 t值
t value4.83** 0.8348 2.06* 8.70** 0.5874 2.26* 1.98* 3.35** 2季间的r
The r between two seasons0.7829** 0.7614** 0.6070** 0.8389** −0.1426 0.9672** 0.8256** 0.9190** *: P<0.05; **: P<0.01. 表 8 稻米品质间的相关系数
Table 8 Correlation coefficients between rice quality
年份
Year品质
QualityX2 X3 X4 X5 X6 X7 X8 2021 X1 −0.4187 −0.3750 0.5388* −0.2121 0.2512 0.3361 0.4169 X2 0.6507** −0.4280 0.0203 0.0013 −0.0795 −0.1017 X3 −0.5827* −0.3270 −0.1031 −0.0967 −0.2673 X4 0.1597 0.1627 0.0970 0.4628 X5 −0.0366 −0.3051 −0.0199 X6 −0.1711 0.1516 X7 0.6984** 2022 X1 −0.5193* −0.3691 0.3589 −0.3538 −0.4720 0.1445 −0.0558 X2 0.9011** −0.3461 −0.2526 0.1257 −0.4155 −0.3148 X3 −0.2687 −0.1932 −0.0132 −0.4693 −0.5385* X4 −0.1302 −0.4816 −0.4194 0.2741 X5 0.5597* 0.3528 0.0461 X6 0.5052 0.0776 X7 0.1860 合计
TotalX1 0.1417 −0.5446** 0.7118** −0.0389 0.5897** 0.6214** 0.6669** X2 0.2264 0.3196 0.0959 0.6499** 0.4078* 0.5722** X3 −0.5744** −0.2896 −0.4713 −0.5609** −0.5394** X4 0.2111 0.7181** 0.5572** 0.8058** X5 0.2499 0.1333 0.2024 X6 0.8222** 0.9203** X7 0.8566** X1: 长宽比; X2: 垩白度; X3: 垩白粒率; X4: 蛋白质; X5: 直链淀粉; X6: 胶稠度; X7: 精米率; X8: 整精米率。*和**分别表示在P<0.05和P<0.01水平差异显著。X1: length to width ratio; X2: chalkiness; X3: chalky rice rate; X4: protein; X5: amylose; X6: gel consistency; X7: milled rice rate; X8: head rice rate. * and ** indicate significance at P<0.05 and P<0.01 levels, respectively. -
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