Abstract: Agriculture is an agroecosystem with a cybernetic system nature. The low-carbon development of agriculture falls into the category of eco-friendly eco-agriculture. Human direct regulation methods for the low-carbon development of eco-agriculture originate from the exploration of traditional and local farmers, modern interdisciplinary agricultural research, and eco-friendly high-tech industries. To get low-carbon goals, the methods from different sources, which are conducive to adapting and strengthening the natural regulation process, can be investigated for their compatibility, synergy and effectiveness within a specific system. The selected methods can be synthesized and optimized to help form a diversified low-carbon system and a suitable technical package. The top-down stimulation measures introduced by the government and the bottom-up efforts provided by people constitute the human indirect regulation for agroecosystems. These entities need to cooperate to form a social joint force to effectively accelerate the low-carbon development of eco-agriculture to reach the national goal of carbon neutralization.