Abstract: Arbuscular mycorrhizal fungi (AMF) can help host plants in the uptake of nutrients, especially phosphorus (P). Intercropping also can promote use efficiency of P in soils and increase crop yield. In order to study the effects of different P additions and AMF treatments on the absorption and utilization of different forms of P of intercropping corps in red soils, a pot simulation experiment was conducted using the three-compartment method. Under maize-soybean intercropping system, the hyphal compartments were treated with different forms of P-no P (P0), inorganic P (potassium dihydrogen phosphate-IOP50) and organic P (soy lecithin-OP50). Different AMF treatments, including no AMF (NM), inoculation with Funneliformis mosseae (FM), were performed in the root chamber. Intercropped maize and soybean, mono-cropped maize and soybean were planted in the experiment. The results showed that compared with mono-cropping-FM and IOP50 treatment, P uptake in maize increased by 150.2% and P uptake in soybean increased by only 24.5% under intercropping-FM and IOP50 treatment. Except mono-cropped soybean and P0 treatment, FM inoculation significantly decreased available P content in hyphal compartment soils. In addition to soybean mono-cropping-FM treatment, IOP50 application yielded the highest soil available P content under mono-cropping, and the lowest under intercropping conditions. AMF inoculation increased the contents of Ca₂-P, Al-P, Org-P, O-Al-P and Ca₁₀-P to a certain extent, while intercropping significantly increased crop uptake of Ca₂-P and Fe-P in the soil. Under combined intercropping, FM and IOP50, the contents of Ca₂-P, Org-P and O-Al-P in the soil were higher (P < 0.05) compared with other treatments. Correlation analysis showed that Ca₂-P was significantly negatively correlated with P uptake in maize, while O-Al-P was significantly negatively correlated with P uptake in soybean. In conclusion, FM inoculation, P fertilizer application and intercropping all promoted P absorption and accumulation of host crops to a certain extent. Among these, FM, IOP50 and intercropping was the best combination for the promotion of intercropped maize growth, P uptake and increased Ca₂-P, Org-P and O-Al-P of red soil. If applied to Dianchi watershed, it is expected to reduce the loss of P.