猪排泄物恶臭产生与控制的微生物学原理
Microbiological mechanism of swine excreta odor production and control
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摘要: 猪排泄物恶臭的产生是一个涉及众多微生物的复杂过程, 研究排泄物中本土菌群的微生物组成及在恶臭产生中的作用是研制与开发有效恶臭控制技术的前提。本文从猪排泄物中主要本土菌群的组成, 不同菌群在恶臭产生中的作用, 主要恶臭化合物来源, 排泄物的重要恶臭指示物及相关的产生菌, 主要恶臭控制技术的微生物原理等角度综述了近几十年来有关猪排泄物恶臭产生与控制的研究进展。然而, 由于猪粪尿排泄物恶臭成分复杂, 影响因素众多, 且是一个主观性很强的评价指标, 有关恶臭指示物的研究仍需进一步开展和深入。此外, 猪粪尿排泄物中很多微生物是厌氧或兼性厌氧菌, 无法通过普通的平板划线法而获得纯培养, 今后应将传统的微生物厌氧培养方法与现代微生物分子生物学技术结合, 动态了解和掌握不同菌群的生长状态及在恶臭产生过程中的作用。Abstract: The generation of swine excreta odors is a complex microbiological process of incomplete anaerobic degradation of carbohydrates, proteins, and lipid components in wastes. Understanding bacterial genera indigenous to swine excreta and their potential for odorous compound production is essential for developing effective odor control techniques. This review covers available information in recent decades that is related to main bacterial genera indigenous to swine excreta, role of different bacterial genera in odorous compound production, origin of excreta odor, odor indicator and related bacterial genera, and microbiological mechanisms and control techniques. However, due to the makeup and complexity of odorous compounds and the subjectivity of odor evaluation, further research is required on related studies of swine excreta odor indicators. Furthermore, several bacteria genera are anaerobic or facultative anaerobic, and therefore cannot form pure cultures by ordinary streak plate method. Combination of well developed classical anaerobic microbiological techniques with modern molecular techniques, along with dynamic understanding of growth status and mode of odor production by different bacteria genera is critical for successful future studies.