曹光球, 杨梅, 林思祖, 黄燕华. 邻羟基苯甲酸对不同化感型杉木无性系抗氧化酶活性的化感效应[J]. 中国生态农业学报(中英文), 2010, 18(6): 1267-1271. DOI: 10.3724/SP.J.1011.2010.01267
引用本文: 曹光球, 杨梅, 林思祖, 黄燕华. 邻羟基苯甲酸对不同化感型杉木无性系抗氧化酶活性的化感效应[J]. 中国生态农业学报(中英文), 2010, 18(6): 1267-1271. DOI: 10.3724/SP.J.1011.2010.01267
CAO Guang-Qiu, YANG Mei, LIN Si-Zu, HUANG Yan-Hua. Allelopathic effects of salicylic acid on activities of antioxidant enzymes of different allelopathic Chinese fir clones[J]. Chinese Journal of Eco-Agriculture, 2010, 18(6): 1267-1271. DOI: 10.3724/SP.J.1011.2010.01267
Citation: CAO Guang-Qiu, YANG Mei, LIN Si-Zu, HUANG Yan-Hua. Allelopathic effects of salicylic acid on activities of antioxidant enzymes of different allelopathic Chinese fir clones[J]. Chinese Journal of Eco-Agriculture, 2010, 18(6): 1267-1271. DOI: 10.3724/SP.J.1011.2010.01267

邻羟基苯甲酸对不同化感型杉木无性系抗氧化酶活性的化感效应

Allelopathic effects of salicylic acid on activities of antioxidant enzymes of different allelopathic Chinese fir clones

  • 摘要: 自毒作用是导致杉木人工林连栽地力衰退、生产力下降的主要原因之一, 邻羟基苯甲酸是杉木主要自毒物质之一。以筛选的自毒物质忍耐型杉木无性系(01号)和自毒物质敏感型杉木无性系(02号)组培苗为试验材料, 分析邻羟基苯甲酸对2个不同化感型杉木无性系抗氧化酶活性的化感效应, 以期为解决杉木连栽障碍提供一条新的生物途径。研究结果表明, 邻羟基苯甲酸各胁迫浓度对不同化感型杉木无性系SOD活性的化感效应在胁迫10 d、20 d表现为促进效应, 胁迫30 d转变为抑制效应; 2个不同化感型杉木无性系SOD活性化感效应的差异主要从胁迫20 d开始, 01号在胁迫20 d促进效应大于02号, 30 d的抑制效应明显小于02号。各胁迫浓度对2个不同化感型杉木无性系叶片POD及AsA-POD活性的化感效应均表现为促进效应; 胁迫第10 d、20 d时, 各胁迫浓度对01号无性系POD活性的促进效应明显大于02号; 01号无性系在受到胁迫后AsA-POD活性迅速增强, 并在长时间胁迫后仍维持比02号无性系更高的活性; 01号无性系PPO活性在各胁迫浓度下均表现为促进效应, 而02号无性系则随胁迫时间的延长表现为抑制-促进-抑制的变化特点。

     

    Abstract: Autotoxicity is one of the main reasons for land degradation and productivity decline in continuous-planting Chinese fir plantations, and salicylic acid (SA) is one of the Chinese fir autotoxins. In this study, the autotoxins-tolerant clone (No. 01) and autotoxins-sensitive clone (No. 02) of Chinese fir were used as the materials in the stress experiment. The allelopathic effects of SA on antioxidant enzymes of the two Chinese fir clones were analyzed to provide the biological strategies to solve the continuous-planting obstacle in pure Chinese fir forest. The results show that all SA levels promote SOD activity of two clones leaves at 10 d and 20 d, but inhibit it at 30 d. The differences of SA allelopathic effects on SOD activity between two clones become obvious from 20 d after stress. Improving effect of SA at the 20th day on No. 01 is more significant, and inhibiting effect at the 30th day is less significant than those on No. 02. All SA levels promote POD and AsA-POD activities of two clones leaves throughout the whole stress period. Stimulatory effect of SA on POD activity of No. 01 clone is greater than that of No. 02 clone at 10th and 20th days of stress. AsA-POD activity of No. 01 increases rapidly after SA stress and keeps higher after long time stress than that of No. 02. PPO activity of No. 01 clone is increased by all SA levels during the stress period, while that of No. 02 clone is decreased, then increased, and decreased finally with stress time.

     

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