Abstract: The embryoes of F₁ hybrids between 4D nullisomic wheat, as female，and rye，as male，were cuhured after fertilizing 16 to 18 days，and the F₁ plantlets were doubled under the treatment of 0．05% colchieine．The doubled plantlets were then backcrossed with nullisomics oneto two times．thus wheat-rye alien substitution lines could be obtained．There were about 18．5% of monosomic substitution plants and about 24．0% of double monosomic substitution in the B₁F₁．Aline substitution lines 4D／4R were produced by their selfing．Now some of the aline substitution lines have been applied directly to production．