Abstract: Relationship between the definition of agro-ecology and the connotation of China eco-agriculture in China is analyzed,the guiding significance of agro-ecology for the area classification,planning and designing,pattern building and evaluation index determining of eco-agriculture construction in China are expounded systematically.It shows that agro-ecology is the theoretical basis of eco-agriculture construction, and the practices in eco-agriculture construction will promote researches and developments of agro-ecology.