Abstract: Investigations on microorganism in different extent soils polluted by high mineralized waste water in oil field, indicate that heterotrophic bacteria, actinomyces and azotobacter would be restrained when the oil concentration of dry land soil exceeds 0.5% and waste water concentration exceeds 0.1%. The amount of the fungi and yeast are increasing along with the increasing of the oil concentration. When oil concentration of paddy field exceeds 3%, aerobic heterotrophicl bacteria separating cultivated bacterium would be obviously restrained, otherwise when waste water concentration exceeds 0.1%, anaerobic heterotrophic bacteria and actinomyces would be obviously restrained.