利用RAPD与SSR技术进行野生大豆种群内分化的研究

Population differentiation of wild soybean based on the RAPD and SSR analysis

摘要

摘要:利用RAPD和SSR技术对25 ^。N野生大豆种群16个样本进行分子标记，从150多个RAPD 引物中筛选出具有多态性的引物20个，共扩增出146个标记位点，其中具有多态性的有60个，占总位点的40．8％，平均遗传距离为0．1536，杂合度为0．3248。从60对SSR引物中筛选出14对具有多态性的引物，平均遗传距离为0．2209，杂合度为0．6961。聚类分析可将25 ^。N野生大豆16个样本分成5类，表明种群内存在大量的遗传变异。为研究野生大豆种群内及种群间的遗传多态性探索了有效方法，并提出野生大豆核心种质资源的保存及取样对策。

Abstract:Random amplified polymorphic DNA (RAPD)and simple sequence repeat(SSR)analyses were applied to 16 samples of wild soybeans( Glycine sojasieb and Zucc)originated from the region of 25 ^。N．Twenty primers screened with 150 ones yielded 146 RAPD bands which had 60 polymorphic products and 40．8％ of total bands．the average heterozygosity(He)was 0．3248 and the average genetic distance among the 16 samples was 0．1536．Fourteen pairs of soybean SSR primers screened had polymorphism ．the average heterozygosity was 0．6961 and the average genetic distance was 0．2209．Cluster analysis showed there are five small classes divided from the 16 samples and there are some evidence showing genetic variations in the population．The genetic resource is also put forward．