牙鲆群体生化遗传学研究——I．组织特异性研究

Biochemical genetics of paralichthys olivaceus population－I．A study on tissue－specific patterns of allozymes

摘要: 采用聚丙烯酰胺凝胶电泳技术，研究了AAT、EST、SOD、MDH、LDH和ME等位酶在牙鲆Paralichthys olivaceus(T．et S．)心脏、肌肉、鳃、眼晴、肾脏、肝脏、肠、脾、胃组织器官中表达，并分析了各种酶的等位酶位点表达以及酶谱表型。研究结果表明EST、SOD、MDH和ME 4种等位酶在不同组织中表达相同，AAT、LDH 2种等位酶在不同组织中表达差异，可能是受不同基因位点或同一基因位点不同等位基因控制的结果，与各组织完成特有生化代谢活动有关。

Abstract: The allozyme patterns of AAT ，EST ，SOD，MDH，LDH ，and ME in heart，muscle，branchia，eye，kidney，liver，intestine，spleen and stomach of paralichthys olivaceus(T．et S．)were studied by using the assay of vertical slab polyacrylamide gel electrophoresis．The results indicate that four allozymes (EST，SOD，MDH，and ME)show the same expressions of allozyme in different tissues，and two allozymes (AAT，LDH)show the different expressions of allozyme in different tisues ，which may be controlled by diferent gene loci or different alleles in the same gene locus，are closely related to the tisues-specific biochemical metabolism．