七子花组培快繁技术研究初探

Prelim inary study on the tissue culture of Heptacodium miconioides Rehd

摘要: 对七子花幼叶诱导愈伤组织及愈伤组织诱导茎的培养基进行筛选，试验研究Mo、Mn及一些促进生根的植物生长激素对扦插枝生根的影响结果表明，愈伤组织的诱导以用嫩叶进行诱导的MS+0．5mg／kg2，4-D+lmg／kgIBA+0．5mg／kgZT 加lg／kg活性碳的培养基较好，茎的诱导以1／2MS+2mg／kg6一BA+0．2mg／kgNAA 的培养基最佳。七子花扦插繁殖用6-BA 处理成活率增加最明显。

Abstract: The media of tender leaves-induced callus and cal1us-induced buds were screened and the effects of Mo,Mn and some plant hormones on cutting production of Heptacodium miconioides were analyzed． The results show that tender leaves are more beneficial to callus inducement than buds and MS+0．5mg／kg2．4-D+lmg／kglBA+0．5mg／kgZT+lg／kg active carbon is the most advantageous medium to callus inducement：1／2 MS+2mg／kg6-BA+0．2mg／kgNAA is the most profitable medium to buds induction；trea tment of 6-BA is the most useful to cutting production.