黎中宝, 李少菁, 王桂忠, 孔祥会. 锯缘青蟹等位酶的生化遗传研究[J]. 中国生态农业学报(中英文), 2004, 12(2): 61-64.
引用本文: 黎中宝, 李少菁, 王桂忠, 孔祥会. 锯缘青蟹等位酶的生化遗传研究[J]. 中国生态农业学报(中英文), 2004, 12(2): 61-64.
LI Zhong-Bao, LI Shao-Jing, WANG Gui-Zhong, KONG Xiang-Hui. Biochemical genetic analysis of allozymes of mud crab,Scylla serrata[J]. Chinese Journal of Eco-Agriculture, 2004, 12(2): 61-64.
Citation: LI Zhong-Bao, LI Shao-Jing, WANG Gui-Zhong, KONG Xiang-Hui. Biochemical genetic analysis of allozymes of mud crab,Scylla serrata[J]. Chinese Journal of Eco-Agriculture, 2004, 12(2): 61-64.

锯缘青蟹等位酶的生化遗传研究

Biochemical genetic analysis of allozymes of mud crab,Scylla serrata

  • 摘要: 应用聚丙烯酰胺凝胶电泳技术,对锯缘青蟹6个自然群体进行l1种等位酶电泳检测和谱带遗传分析结果表明,所研究的锯缘青蟹22个等位酶位点和30个等位基因中有l5个单态位点,即Ldh-l、Sod-2、Aat-l、Aat-2、Skd-1、Skd-2,Idh-1、Sdh-l、Adh-1、Me-l、Me-3,Mdh-l、Amy-1、Amy-2和Amy-3.而这些位点仅有1个等位基因;有7个移态位点,即Est-l、Est-2(此位点厦门锯缘青蟹为单态位点),Est-3、Sod-l,Me-2、Mdh-2(此位点厦门锯缘青蟹为多态位点)和Mdl-3,而这些位点有2~3个等位基因。6个锯缘青蟹自然群体所有位点中共享大多数常见等位基因,其生化遗传非常相似.

     

    Abstract: Allozyme was investigated using the assay of vertical slab polyacrylamide gel electrophoresis in Scylla serrata.Eleven enzymes presum ably encoded by 22 allozyme loci and 30 alleles are scored in S.serrata,the monomorphic loci with one alide are Ldh-1,Sod-2,Aat-l,Aat-2,Skd-l,Skd-2,Idh-l,Sdh-l,Adh-l,Me-l,Me-3, Mdh-l,Amy-l,Amy-2.amd Amy-3;and 7 loci with 2~3 alleles are polymorphic,they are Est-l,Est-2(the monomorphic locus in Xiamen population),Est-3,Sod-l,Me-2,Mdh-2(the polymorphic locus in Xiamen population),and Mdh-3.S.serata in six samples shares nlost common alleles in all1oci,their biochemical genetic are very similar.

     

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