Abstract: The Jojoba plantlets were in vitro treated by the subculture medium containing a suitble concentration of inositol and the result shows that it has a significant promotion to the shoots differentiation and leaf growth. 1～3.5mg/L of Silver nitrate significantly affects the shoots proliferation, but the plantlet height unremarkably. Exorbitant concentration of silver nitrate inhibits the plantlets growth in a certain extent,the best concentration is 3mg/L. A very significant effect on shoots growth and proliferation can be attained by using 500mg/L of inositol together with 3mg/L of Silver nitrte and the proliferation coefficient is 10.9.