Abstract: The successional regulation of agro-ecosystem in Shihezi Oasis along Manas River was analyzed. It shows that oasis agricultural productivity is in the process of nomal succession and the relevant ecology environment becomes retrogressive succession with more technique inputing and market opening. Human's activity is the essential cause of oasis succession and the environment is the key to accelerating the process of oasis succession, among which the distribution and using pattern of water resource are the predominant factor. Positive succession should be based on the temporal and spatial distribution and the coordinated utilization of oasis resource factors on the agro-ecosystem along Manas River.