Abstract: The effects of long-term located fertilization on the available boron content of soil were studied.The results show that the available boron contents in 0～20cm soil layers of the six different fertilization treatments including N ,NP,NPK,N+organic fertilizer,N+straw and no fertilizer show a direct correlation to the years of the localized fertilizer study with the coefficient r=0.8094～0.8847.The different fertilization treatments all give an increased available boron contents,which are 2.9～4.7 times of the basic values of the experiments, and come in the order of N+organic fertilizer＞NP＞N+straw＞N＞NPK＞no f ertilizer.Fertilization combined with NPK helps to win a relatively higher yield,in addition,less available boron is found to be accumulated.Farming and fertilization are the key factors affecting the accumulation of available boron in soil.