Isolation and enzymatic characteristics of cellulase producing microbial community

A cellulase producing microbial community (MO) was isolated from rotted beanstalk compost. When the community was cultured at pH 8.0 and 50 ℃, maximal CMCase activity (1.361 1 IU) is observed in 90 hours. The optimal conditions for enzymatic reaction are 60 ℃ at pH 7.0. High heat and pH stability is observed at temperature lower than 60 ℃ and pH 3～8. CMCase activity of cellulase can reach 2.13 IU at optimal reaction point. Growth kinetics indicate some relationships among pH, CMCase activity and weight loss rate in different growth phases of microbial community. Through cellulase analysis for zymogram via native-PAGE, seven activity bands are observed. This suggests that more than one strain is responsible for the production of enzyme in MO.