Starch Gel and Tissue Culture:II. Synchronized Effects of Corn Starch Gel Medium on Rooting Induction of Malus Zumi and M26

Malus zumi and M26 dwarfing stocks for apple trees were cultured in agar medium and corn starch gel medium(CSM),respectively.The results showed that the CSM not only increased rooting rate in of the two species,but also had synchronized effests on rooting induction.For example,the rooting rate in CSM reached 85-98%,23-30% higher than that in agar medium,and the rooting differentiation was completed in about 2 to 3 days,which was also shorter than that in agar medium.HPLC analysis of free amino acids of M. zumi demonstrated that the CSM inhabited the increase of the content of Arg,Val,Phe,Leu,ORn,and stimulated further reduction of other free amino acids in rooting culture.